Genome stitched together by hand

Scientists have succeeded in stitching together an entire bacterial genome, creating in the lab the full set of instructions needed to make a living thing. The stage is now set for the creation of the first artificial organism — and it could be achieved within the year.

The genome for the pathogenic bacterium Mycoplasma genitalium was made in the laboratory by Hamilton Smith and his colleagues at the J. Craig Venter Institute in Rockville, Maryland. The genome has 582,970 of the fundamental building blocks of DNA, called nucleotide bases, making it more than a factor of ten longer than the previous-longest stretch of genetic material created by chemical means.

Now the team at the Venter institute, which includes the institute’s founder, genomics pioneer Craig Venter, will aim to discover whether cells can be ‘booted up’ into action when loaded with this genetic programme. “This is the next step and we are working on it,” says Smith.

Venter and his colleagues have already managed to transplant the DNA from one bacteria into another, making it change species (see Genome transplant makes species switch/news070625-9). These bacteria were closely related to M. genitalium. If the transplant can be repeated with a man-made genome adapted from M. genitalium, the result could qualify as the first artificial life form (see 'What is artificial life?').

http://www.nature.com/news/2008/080124/full/news.2008.522.html